Alimentation / Biotechnologies

Non-specific endo-1,3(4) beta-Glucanase (Clostridium thermocellum)

Référence : E-LICACT

Non-specific endo-1,3(4) beta-Glucanase (Clostridium thermocellum)

High purity recombinant Non-specific endo-1,3(4)-Beta-Glucanase (Clostridium thermocellum) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

EC 3.2.1.6
CAZy Family: GH16
CAS: 62213-14-3 


endo-1,3(4)-beta-glucanase; 3(or 4)-beta-D-glucan 3(4)-glucanohydrolase

Recombinant: Catalytic domain of LicA from Clostridium thermocellum. 
In 50% glycerol.
Supplied at ~ 1,250 U/mL. 

Specific activity
~ 186 U/mg (40°C, pH 6.5 on barley ?-glucan); 
~ 395 U/mg (60°C, pH 6.5 on barley ?-glucan); 
~ 7.2 U/mg (40°C, pH 6.5 on CM-curdlan).

Stability: > 2 years at 4°C.

Unit definition:
One Unit of glucanase activity is defined as the amount of enzyme required to release one micromole of glucose reducing-sugar equivalents per minute from barley Beta-glucan (5 mg/mL) in sodium phosphate buffer (100 mM) pH 6.5 at 40°C.

Specificity:
endo-hydrolysis of (1,3)- or (1,4)-linkages in Beta-D-glucans when the glucose residue whose reducing group is involved in the linkage to be hydrolysed is itself substituted at C-3. Substrates include laminarin, lichenin and cereal D-glucans.

Applications:
Applications in carbohydrate and biofuels research and in the food and feeds industry.


Produits associés :


Ces données vous sont présentées lors de la dernière mise à jour de juillet 2017.
Elles sont susceptibles d'évoluer, merci de nous demander la dernière notice technique en cours.


Non-specific endo-1,3(4) beta-glucanase, Non-specific endo-1,3(4) beta-Glucanase (Clostridium thermocellum)
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Conditionnement :
5 000 unités
Prix et devis sur simple demande
Mail : info@libios.fr
Tél : 04 74 13 03 02
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  Non-specific endo-1,3(4) beta-Glucanase (Clostridium thermocellum)

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