MDA-TBARS
Reference : KB03016
This method, is convenient to determine the relative lipid peroxide content of samples, including plasma, serum, cell culture supernatants and urine samples.
Multi-unsaturated lipids, are most likely to form peroxides, and so they are the most reactive lipids in the TBARs assay.
TBARs assay is commonly used to compare one set of samples to another.
Lipid oxidation is reported to increase with age and correlates with some clinical features of cardiovascular disease, ischemia/reperfusion and cerebrovascular disorders among others.
Lipid peroxidation is a well-defined mechanism of cellular damage. Lipid peroxides are unstable indicators of oxidative stress in cells. They decompose to form more reactive compounds such as Malondialdehyde (MDA) and 4-hydroxynonenal (4-HNE), natural biproducts of lipid peroxidation. In vitro, a wide array of pro-oxidant agents can modificate lipids by oxidation and, in vivo, it occurs during aging and in certain disease conditions.
Nowadays, one of the most widely accepted assays for oxidative damage is the measurement of the end products of lipid peroxidation. Specifically, the most generally used test, in the appreciation of the role of oxidative stress in disease, is the MDA assay. During the radical induced decomposition of polyunsaturated fatty acids, MDA is one of the several products. Most often, MDA assay uses its reactivity at high temperature and low pH, towards thiobarbituric acid. Although, this reaction is very sensitive, its specificity, is still a matter of debate. Furthermore, it persit even with the improvement of pre-analytical (sampling, preservatives) and analytical stages (fluorescence, HPLC). At present, the concept of “thiobarbituric acid reactive substances” (TBARS) have merged and progressively replaced the initial malondialdehyde assay.
The Thiobarbituric Acid Reactive Substances (TBARS) Assay Kit is a tool for the direct quantitative measurement of MDA in biological samples. The samples containing MDA or the MDA standards react with TBA. After incubation, the user can read samples and standards, both spectrophotometrically or fluorometrically. Finally, the user can calculate the concentration of MDA in the samples by comparing with the MDA standard curve.
The documents available in this section are provided for information only and are subject to change. Please contact us for the latest updates.
If you have just received a kit or product without technical instructions in paper format, this means that the instructions have been dematerialized.
In this case, please download it and refer only to the version you can download from the product page of the manufacturer's website, or by scanning the QR code present on the product.
Regarding any certificates of analysis, safety data sheets or spreadsheets, please contact us to obtain the latest versions.
If you have any questions, our team is at your disposal.
Specifications
Reference | Packaging | |
---|---|---|
KB03016-100 | 100 tests (96 puits/wells format) | |
KB03016-200 | 200 tests (96 puits/wells format) | |
KB03016-400 | 400 tests (96 puits/wells format) |