Catalase
Reference : KB03012
This Catalase Activity Assay Kit consist on a reaction giving rise to a compound that forms a complex with the chromogen. This reaction produces a purple color directly proportional to catalase activity that can be determined by means of a simple and fast spectrophotometrical measure.
Information
Hydrogen peroxide is a reactive oxygen specie (ROS). It is a toxic product of normal aerobic metabolism and pathogenic ROS production, involving oxidase and superoxide dismutase reactions. Hydrogen peroxide is poisonous to eukaryotic cells. Particularly, in high doses, it can initiate oxidation of DNA, lipids, and proteins, which can lead to mutagenesis and cell death.
On the other hand, catalase is an antioxidant enzyme omnipresent in mammalian and non-mammalian cells. Particularly, the reaction that they catalyze is the destruction of hydrogen peroxide by dismutation. Eukaryotic catalases are heme enzymes, which are present in the liver, kidney, and erythrocytes in high concentrations. However, the lowest concentrations are in the connective tissues.
This enzyme catalyzes the reaction that consumes hydrogen peroxide, transforming it into water and oxygen. Since it is difficult to directly measure hydrogen peroxide in biological samples, the determination of these detoxifying enzymes has substituted this procedure. In addition, Catalase activity levels are also related to antioxidant capacity.
The documents available in this section are provided for information only and are subject to change. Please contact us for the latest updates.
If you have just received a kit or product without technical instructions in paper format, this means that the instructions have been dematerialized.
In this case, please download it and refer only to the version you can download from the product page of the manufacturer's website, or by scanning the QR code present on the product.
Regarding any certificates of analysis, safety data sheets or spreadsheets, please contact us to obtain the latest versions.
If you have any questions, our team is at your disposal.
Specifications
Reference | Packaging | |
---|---|---|
KB03012-100 | 100 tests (96 puits/wells format) | |
KB03012-200 | 200 tests (96 puits/wells format) | |
KB03012-400 | 400 tests (96 puits/wells format) |