Endo-cellulase (high sensitivity) - Enzyme activity assay kit
Reference : K-CellG5
Shipping temperature: ambient
Storage temperature:
- Short term stability: 2-8oC,
- Long term stability: see individual component labels
Assay format: spectrophotometer, auto-analyser
Detection method: absorbance - 400
Signal response: increase
Total assay time: ~ 10 min
Detection limit: 1,2 x 10-3 U/ml
Reproducibility (%): ~ 3%
Stability: > 2 years under recommended storage conditions
The CellG5 assay reagent for the measurement of endo-cellulase (endo-1,4-β-glucanase) contains two components :
1) 4,6-O-(3-ketobutylidene)-4-nitrophenyl-β-D-cellopentaoside (BPNPG5)
2) thermostable β-glucosidase.
The ketone blocking group prevents any hydrolytic action by the β-glucosidase on BPNPG5. Incubation with an endo-cellulase generates a non-blocked colourimetric oligosaccharide that is rapidly hydrolysed by the ancillary β-glucosidase. The rate of formation of 4-nitrophenol is therefore directly related to the hydrolysis of BPNPG5 by the endo-cellulase. The reaction is terminated and the phenolate colour is developed on addition of Tris buffer solution (pH 9.0).
The CellG5 assay represents a huge step forward in the methodology for the measurement of cellulase that traditionally relied on substrates such as CM-cellulose, Avicel, cellooligosaccharides, filter paper or dyed polysaccharides including CMC Congo red or cellulose azure.
Novel method
- Very cost effective
- All reagents stable for > 4 years after preparation
- Completely specific for cellulase (endo-1,4-glucanase)
- Generally applicable and highly sensitive
- Simple format. Well suited to automation
- Standard included
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Specifications
Reference | Packaging | |
---|---|---|
K-CELLG5-4V | 120 / 480 tests (manuel/manual) - 240 tests (automate/auto-analyser) |