Pectate lyase (Aspergillus sp.)
- In 50% aqueous glycerol
Reference : E-PCLYAN
CAS Number : 9015-75-2
Shipping temperature: ambient
Storage temperature: <-10°C
Formulation: in 50% (v/v) glycerol
Physical form: solution
EC 4.2.2.2
CAZy Family: PL1
CAS: 9015-75-2
Synonyms: pectate lyase; -α-D-galacturonan lyase
Source: Aspergillus sp.
Highly purified
Molecular weight: 45,000
Concentration: supplied at ~ 1,400 U/mL
Specific activity:
~ 180 U/mg (40°C, pH 8.0 on polygalacturonic acid)
Unit definition:
One Unit of pectate lyase activity is defined as the amount of enzyme required to release one micromole of galacturonic acid from polygalacturonic acid (2.5 mg/mL) per min in Tris.HCl buffer (50 mM), pH 8,0 at 40°C
Specificity:
Eliminative cleavage of (1,4)-α-D-galacturonan to give oligosaccharides with 4-deoxy-alpha-D-galact-4-enuronosyl groups at their non-reducing ends
Temperature optima: 55°C
pH optima: 8
Stability: > 4 years at 4°C or - 10°C
- High purity and stability
- Suitable for pectin identification according to USP method [Pectin 9000-69-5]; Pectin Identification Procedure. Applications in carbohydrate and biofuels research
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Specifications
Reference | Packaging | |
---|---|---|
E-PCLYAN | 7 000 unités (units) dans 50% glycérol |