Exo-α-sialidase (Clostridium perfringens)
Reference : E-SIALCP
CAS Number : 9001-67-6
Shipping temperature: ambient
Storage temperature: 2-8oC
Formulation: in 20 mM tris buffer containing 50 mM NaCl2 and 5 mM EDTA
Physical form: suspension
EC 3.2.1.18
CAZy Family: GH33
CAS: 9001-67-6
Synonyms: exo-α-sialidase; acetylneuraminyl hydrolase
Source: Clostridium perfringens
Molecular weight: 43,600
Concentration: supplied at ~ 500 U/mL
Specific activity:
~ 140 U/mg (37°C, pH 7.0 on pNP-α-D-N-acetylneuraminic acid)
Unit definition:
One unit of sialidase activity is defined as the amount of enzyme required to release one micromole of p-nitrophenol per minute from pNP-alpha-D-N-acetylneuraminic acid (1 mM) in sodium phosphate buffer (100 mM), pH 7,0 at 37°C
Specificity:
Hydrolysis of unbranched, non-reducing terminal α-2,3-linked, α-2,6-linked >> α-2,8-linked N-acetylneuraminic acid (NANA; Neu5Ac) residues from glycoproteins and oligosaccharides of glycoconjugates
Temperature optima: 37°C
pH optima: 7
Stability: >2 years at 4°C
- High purity and stability
- Clinical chemistry, glycobiology and carbohydrate research
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Specifications
Reference | Packaging | |
---|---|---|
E-SIALCP | 5 unités (units) |