Amyloglucosidase (Aspergillus niger) - Glycerol free
Reference : E-AMGDFNG
CAS Number : 9032-08-0
Shipping temperature: ambient
Storage temperature: 2-8°C
Formulation: in buffered solution
Physical form: solution
EC 3.2.1.3
CAZy Family: GH15
CAS: 9032-08-0
Synonyms: glucan 1,4-α-glucosidase; 4-αa-D-glucan glucohydrolase;glucoamylase
Source: Aspergillus niger
Recombinant from Aspergillus niger
Molecular weight: 143,500
Specific activity:
~ 36 U/mg (40°C, pH 4,5 on soluble starch)
~ 400 U/mL on p-nitrophenyl Beta-maltoside
~ 6,520 U/mL on soluble starch
Unit definition:
One Unit of amyloglucosidase activity is defined as the amount of enzyme required to release one µmole of glucose reducing-sugar equivalents per minute from soluble starch (10 mg/mL) at pH 4,5 at 40°C
Specificity:
Hydrolysis of terminal (1,4)-linked α-D-glucose residues successively from non-reducing ends of the chains with release of β-D-glucose
Temperature optima: 70°C
pH optima: 4
Stability: > 4 years 4°C
The preparation is free of glycerol so can be used in TDF procedures where glycerol is used as an internal standard (e.g. AOAC Method 2001.03/AACC Method 32-41.01; the Matsutani Method).
- High purity and stability
- This enzyme is recommended for use in Total Dietary Fibre analytical procedures and the Total Starch test method.
The preparation is free of glycerol so can be used in TDF procedures where glycerol is used as an internal standard (e.g. AOAC Method 2001.03/AACC Method 32-41.01; the Matsutani Method).
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Specifications
Reference | Packaging | |
---|---|---|
E-AMGDFNG-20ML | 20 mL |